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Kern et al., 2014)
Without enough thiamine, the vagus nerve cannot function in the normal way
Cell were washed and placed in normal Ringers buffer (160 mM NaCl, 4.5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES, pH 7.4) or Low Ca 2+ Ringers where CaCl 2 was omitted and 1 mM Ethylenediaminetetraacetic acid (EDTA) added
500 ampoules (50 x 10 ampoules of 1 ml injection solution as a bundle pack)
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